PAL2NAL is a program that converts a multiple sequence alignment of proteins and the corresponding DNA (or mRNA) sequences into a codon alignment. 

http://www.bork.embl.de/pal2nal/

map the nucleotide sequences to the multiple alignment of amino acid sequences.

import tempfilefrom Bio import SeqIOimport subprocesspal2nal = 'C:\\Users\\dell\\Desktop\\pal2nal.v14\\pal2nal.pl'p = subprocess.check_output('perl %s %s %s -codontable 1 -output fasta' % (pal2nal, alnFile, nucFile), stderr = subprocess.PIPE, shell = True)tmf = tempfile.TemporaryFile()tmf.write(p)tmf.seek(0)seq = list(SeqIO.parse(tmf, 'fasta'))tmf.close()

简单用了几个模块将pal2nal的进程在python里封装一下。suprocess里面的PIPE不是很懂，大概就是定义输出输入流吧。用临时文件存储输出，再解析临时文件。